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(a) Small molecules inhibit STAT3/phosphopeptide (p1068) binding. STAT3 phosphopeptide binding was measured with established SPR protocols.21 (b) Kasumi cells were treated with G-CSF and then with inhibitors. Cells were analyzed for phosphorylated STAT3 (pSTAT3). (c) Apoptosis was measured 24 h after treatment of AML cells with STAT3 inhibitors.
