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Published in final edited form as: Trends Analyt Chem. 2018 Nov 24;120:115322. doi: 10.1016/j.trac.2018.11.020

Figure 6. — PDO was isolated from a non-small cell lung cancer patient (UK022) tumor xenograft in Matrigel and subsequently grown as M3DB cultures (example 20x image shown) in [¹³C₆]-Glc+DMEM medium ± 10 µM selenite (SeO₃) for 48 hr. Atom-resolved tracing from [¹³C₆]-Glc to glycolytic, Krebs cycle, and gluconeogenic (GNG) metabolites is shown along with the ¹³C labeling patterns of these metabolites in response to SeO₃. Also shown are the SeO₃-induced changes in ¹³C labeling patterns of the products of PPP, purine (PUR)/pyrimidine (PYR) synthesis, and glutathione (GSH) synthesis. The numbers in X-axis represent the number of ¹³C atoms (isotopologues) in each metabolite while “Ring” in ATP and UTP indicates the sum of all ¹³C-nucleobase isotopologues. denote GNG and anaplerotic inputs into the Krebs cycle mediated by PCB, respectively. ●: ¹²C; : PDH-, PCB-, or malic enzyme (ME) initiated Krebs cycle reactions, respectively; : GNG; F1,6BP: fructose-1,6-bisphosphate; PEP: phosphoenolpyruvate; PRPP: phosphoribosylpyrophosphate; PEPCK: PEP carboxykinase; MDH: malate dehydrogenase; AST: aspartate amino transferase. All other symbols and abbreviations are as in Fig. 5.

Inline graphic — PDO was isolated from a non-small cell lung cancer patient (UK022) tumor xenograft in Matrigel and subsequently grown as M3DB cultures (example 20x image shown) in [¹³C₆]-Glc+DMEM medium ± 10 µM selenite (SeO₃) for 48 hr. Atom-resolved tracing from [¹³C₆]-Glc to glycolytic, Krebs cycle, and gluconeogenic (GNG) metabolites is shown along with the ¹³C labeling patterns of these metabolites in response to SeO₃. Also shown are the SeO₃-induced changes in ¹³C labeling patterns of the products of PPP, purine (PUR)/pyrimidine (PYR) synthesis, and glutathione (GSH) synthesis. The numbers in X-axis represent the number of ¹³C atoms (isotopologues) in each metabolite while “Ring” in ATP and UTP indicates the sum of all ¹³C-nucleobase isotopologues. denote GNG and anaplerotic inputs into the Krebs cycle mediated by PCB, respectively. ●: ¹²C; : PDH-, PCB-, or malic enzyme (ME) initiated Krebs cycle reactions, respectively; : GNG; F1,6BP: fructose-1,6-bisphosphate; PEP: phosphoenolpyruvate; PRPP: phosphoribosylpyrophosphate; PEPCK: PEP carboxykinase; MDH: malate dehydrogenase; AST: aspartate amino transferase. All other symbols and abbreviations are as in Fig. 5.