Figure 7.

Native PAGE of MBP-fused AMHR2 ECD with titration of rhAMH. (A) Complex formation between WT MBP-fused AMHR2 ECD with rhAMH was determined by basic Native PAGE run at 4°C for 4.5 hours at 120 V. First lane of each gel shows the control of receptor alone at the concentration used in the highest ratio of 1:32 during the titration. rhAMH is not visible on basic native PAGE. Complex and MBP-fused AMHR2 ECD alone bands are noted by labeled arrow. rhAMH was incubated with MBP-fused AMHR2 ECD by increasing molar ratios from 1:2 to 1:32 excess receptor. (B) Bands denoted by boxes in (A) and (C) were excised, electro-eluted, and analyzed by SDS-PAGE under reducing conditions C-K). Complex formation test of mutant MBP-fused AMHR2 ECD with rhAMH.