Figure 3.

SIK inhibition enhances FSH actions in primary rat GCs. Rat GCs were treated with vehicle, FSH, FSH plus (A) HG-9-91-01 (HG) or (B) MRT67307 (MRT), or SIK inhibitors alone. Cyp19a1 mRNA levels were quantified 48 hours later and expressed as relative expression (RE) to Rpl19. (C) Lentivirus was used to deliver an empty luciferase reporter or a reporter carrying the ovarian Cyp19a1 promoter. Cells were treated with vehicle, FSH, FSH plus HG, or HG alone 48 h after the addition of the virus. Luciferase activity was quantified 48 h after the initiation of treatments. (D) Rat GCs were treated with vehicle, FSH, FSH plus HG or MRT, or SIK inhibitors alone. Estradiol concentration in the media was determined by enzyme-linked immunosorbent assay 48 hours after the initiation of treatments. Different letters represent significant differences between groups, 1-way ANOVA followed by Tukey, P < .05, N ≥ 5. **P = .0012, t-test. nd, non-detectable.