Fig. 5. TLS polymerases as a gap suppression mechanism in cancer that can be resensitized by using the TLSi.

(A and B) Representative images and quantification of colony formation after dose-dependent treatment with ATRi (μM) and WEE1i (nM) alone or in combination with 20 μM TLSi across the different cell lines. (C) Model summarizing that TLS polymerases are a replication stress avoidance mechanism in cancer. Experiments were performed in biological triplicate. Bars represent the means ± SD. Statistical analysis was performed according to two-tailed Mann-Whitney test. All P values are described in the “Statistical methods.”