Fig. 6. Clonal architecture and evolution of PDXs in CRC.

(A) Experimental procedure for tumor harvesting and PDX development via single-cell suspension. All samples were derived from tumor tissue resected at the time of surgery. Engraftment procedures uniformly involve creation of single-cell suspension at each step of harvest and subsequent PDX development to maintain the clonal diversity of each sample. All PDX samples were collected after two passages when the PDXs were considered established. (B) Summary of the clones seen in each PDX compared with matched parental tumor sample and number of clones observed in patient. (C) Clonal selection from parental tumor to its matched PDX. Numbers above the arrows represent the number of clones present in each sample. These data demonstrate that most PDXs fail to accurately recapitulate the clonal architecture of their parental tumors.