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. 2020 May 29;9:e56048. doi: 10.7554/eLife.56048

Figure 1. Suppression of the growth defects of a L. monocytogenes ΔgpsB mutant by reoM and reoY mutations.

(A–B) Effect of suppressor mutations on growth of the ΔgpsB mutant. Growth of L. monocytogenes strains EGD-e (wt), LMJR19 (ΔgpsB), shg8 (ΔgpsB reoY H87Y), shg10 (ΔgpsB reoY TAA74) and shg12 (ΔgpsB reoM RBS mutation) in BHI broth at 37°C (A) and 42°C (B). (C–D) Effect of ΔreoM and ΔreoY deletions on growth of L. monocytogenes. Growth of L. monocytogenes strains EGD-e (wt), LMJR19 (ΔgpsB), LMSW30 (ΔreoM), LMSW32 (ΔreoY), LMJR137 (ΔgpsB ΔreoM) and LMJR120 (ΔgpsB ΔreoY) in BHI broth was recorded at 37°C (C) and 42°C (D). All growth experiments were performed three times and average values and standard deviations are shown.

Figure 1.

Figure 1—figure supplement 1. Overexpression of reoM but not reoY affects growth of the L. monocytogenes ΔgpsB mutant.

Figure 1—figure supplement 1.

(A) Growth of L. monocytogenes strains EGD-e (wt), LMJR19 (ΔgpsB) and LMJR106 (ΔgpsB+reoY) in BHI broth containing 1 mM IPTG at 37°C. (B) Growth of strains EGD-e (wt), LMJR19 (ΔgpsB), LMJR102 (wt+reoM) and LMJR96 (ΔgpsB+reoM) in BHI broth containing 1 mM IPTG at 37°C. The experiments were performed twice and one representative result is shown.
Figure 1—figure supplement 2. Effect of reoM and reoY deletions on cell morphology.

Figure 1—figure supplement 2.

Strains EGD-e (wt), LMJR19 (ΔgpsB), LMSW30 (ΔreoM), LMSW32 (ΔreoY), LMJR137 (ΔgpsB ΔreoM), and LMJR120 (ΔgpsB ΔreoY) were grown in BHI broth at 37°C to mid-logarithmic growth phase and cell lengths were measured after nile red staining and fluorescence microscopy. Diagrams show the effect of reoM (A) and reoY (B) deletions on cell lengths of wild type and ΔgpsB mutant cells as cell length frequency distributions determined on 300 cells per strain from a single culture. Results of one out of three experiments are shown. (C) Scanning electron microscopy of the same strains as in panel A and B. Strains were grown to mid-logarithmic growth phase in BHI at 37°C and subjected to chemical fixation and subsequent electron microscopy as described in the experimental procedures section. Scale bar: 2 µm.