Table 2. Plasmids and strains used in this study.
| Name | Relevant characteristics | Source*/reference |
|---|---|---|
| Plasmids | ||
| pIMK3 | Phelp-lacO lacI neo | Monk et al., 2008 |
| pMAD | bla erm bgaB | Arnaud et al., 2004 |
| pUT18 | bla Plac-cya(T18) | Karimova et al., 1998 |
| pUT18C | bla Plac-cya(T18) | Karimova et al., 1998 |
| pKT25 | kan Plac-cya(T25) | Karimova et al., 1998 |
| p25-N | kan Plac-cya(T25) | Claessen et al., 2008 |
| pJR127 | bla erm bgaB ΔclpC (lmo0232) | Rismondo et al., 2017 |
| pSH246 | bla erm bgaB ΔgpsB (lmo1888) | Rismondo et al., 2016 |
| pJR68 | bla erm bgaB ΔmurZ (lmo2552) | Rismondo et al., 2017 |
| pJR71 | Phelp-lacO-murZ lacI neo | Rismondo et al., 2017 |
| pJR65 | Phelp-lacO-reoM lacI neo | this work |
| pJR70 | Phelp-lacO-reoY lacI neo | this work |
| pJR83 | bla erm bgaB ∆reoY (lmo1921) | this work |
| pJR101 | kan Plac-cya(T25)-reoM | this work |
| pJR102 | kan Plac-reoM-cya(T25) | this work |
| pJR103 | bla Plac-reoM-cya(T18) | this work |
| pJR104 | bla Plac-cya(T18)-reoM | this work |
| pJR109 | kan Plac-cya(T25)-reoY | this work |
| pJR111 | bla Plac-cya(T18)-reoY | this work |
| pJR116 | kan Plac-cya(T25)-murA | this work |
| pJR117 | kan Plac-murA-cya(T25) | this work |
| pJR118 | bla Plac-murA-cya(T18) | this work |
| pJR119 | bla Plac-cya(T18)-murA | this work |
| pJR121 | bla Plac-reoY-cya(T18) | this work |
| pJR126 | bla erm bgaB ∆reoM (lmo1503) | this work |
| pSW29 | Phelp-lacO-reoM T7A lacI neo | this work |
| pSW30 | Phelp-lacO-reoM T7D lacI neo | this work |
| pSW36 | bla erm bgaB ΔprkA (lmo1820) | this work |
| pSW37 | bla erm bgaB ΔprpC (lmo1821) | this work |
| pSW38 | Phelp-lacO-prkA lacI neo | this work |
| pSW39 | Phelp-lacO-prpC lacI neo | this work |
| pSW43 | kan Plac-cya(T25)-clpC | this work |
| pSW44 | kan Plac-cya(T25)-clpP | this work |
| pSW45 | kan Plac- clpC-cya(T25) | this work |
| pSW46 | kan Plac-clpP-cya(T25) | this work |
| pSW47 | bla Plac-clpC-cya(T18) | this work |
| pSW48 | bla Plac-clpP-cya(T18) | this work |
| pSW49 | bla Plac-cya(T18)-clpC | this work |
| pSW50 | bla Plac-cya(T18)-clpP | this work |
| pSW55 | Phelp-lacO-reoM R66A lacI neo | this work |
| pSW56 | Phelp-lacO-reoM R70A lacI neo | this work |
| pSW58 | Phelp-lacO-reoM R57A lacI neo | this work |
| pSW59 | Phelp-lacO-reoM R62A lacI neo | this work |
| B. subtilis strains | ||
| 168 | wild type, lab collection | |
| BKE00860 | ΔclpC | Koo et al., 2017 |
| BKE22180 | ΔgpsB | Koo et al., 2017 |
| BKE22580 | ΔypiB (reoY) | Koo et al., 2017 |
| BKE27400 | ΔyrzL (reoM) | Koo et al., 2017 |
| L. monocytogenes strains | ||
| EGD-e | wild-type, serovar 1/2a strain | Glaser et al., 2001 |
| LMJR19 | ΔgpsB (lmo1888) | Rismondo et al., 2016 |
| LMJR104 | ∆murZ (lmo2552) | Rismondo et al., 2017 |
| LMJR116 | attB::Phelp-lacO-murA lacI neo | Rismondo et al., 2017 |
| LMJR123 | ΔmurA (lmo2526) attB::Phelp-lacO-murA lacI neo | Rismondo et al., 2017 |
| LMJR138 | ΔclpC (lmo0232) | Rismondo et al., 2017 |
| shg8 | ΔgpsB reoY H87Y | this work |
| shg10 | ΔgpsB reoY TAA74 | this work |
| shg12 | ΔgpsB reoM RBS mutation | this work |
| LMJR96 | ∆gpsB attB::Phelp-lacO-reoM lacI neo | pJR65 → LMJR19 |
| LMJR102 | attB::Phelp-lacO-reoM lacI neo | pJR65 → EGD-e |
| LMJR106 | ∆gpsB attB::Phelp-lacO-reoY lacI neo | pJR70 → LMJR19 |
| LMJR120 | ΔgpsB ΔreoY | pJR83 ↔ LMJR19 |
| LMJR137 | ΔgpsB ΔreoM | pJR126 ↔ LMJR19 |
| LMJR171 | ΔclpC ΔmurZ | pJR127 ↔ LMJR104 |
| LMSW30 | ΔreoM (lmo1503) | pJR126 ↔ EGD-e |
| LMSW32 | ΔreoY (lmo1921) | pJR83 ↔ EGD-e |
| LMSW50 | ΔclpC ΔreoM | pJR127 ↔ LMSW30 |
| LMSW51 | ΔclpC ΔreoY | pJR127 ↔ LMSW32 |
| LMSW52 | ΔreoM attB::Phelp-lacO-reoM T7A lacI neo | pSW29 → LMSW30 |
| LMSW53 | ΔreoM attB::Phelp-lacO-reoM T7D lacI neo | pSW30 → LMSW30 |
| LMSW57 | ΔreoM attB::Phelp-lacO-reoM lacI neo | pJR65 → LMSW30 |
| LMSW72 | ΔreoM attB::Phelp-lacO-reoM T7A lacI neo ΔclpC | pJR127 ↔ LMSW52 |
| LMSW76 | ΔprpC prkA* | pSW37 ↔ EGD-e |
| LMSW80 | attB::Phelp-lacO-prkA lacI neo | pSW38 → EGD-e |
| LMSW81 | attB::Phelp-lacO-prpC lacI neo | pSW39 → EGD-e |
| LMSW83 | ΔprpC attB::Phelp-lacO-prpC lacI neo | pSW37 ↔ LMSW81 |
| LMSW84 | ΔprkA attB::Phelp-lacO-prkA lacI neo | pSW36 ↔ LMSW80 |
| LMSW89 | ΔprkA attB::Phelp-lacO-prkA lacI neo ΔreoM | pJR126 ↔ LMSW84 |
| LMSW90 | ΔprkA attB::Phelp-lacO-prkA lacI neo ΔreoY | pJR83 ↔ LMSW84 |
| LMSW91 | ΔprkA attB::Phelp-lacO-prkA lacI neo ΔclpC | pJR127 ↔ LMSW84 |
| LMSW117 | ΔreoM ΔreoY | pJR126 ↔ LMSW32 |
| LMSW118 | ΔreoY ΔmurZ | pJR68 ↔ LMSW32 |
| LMSW119 | ΔreoM ΔmurZ | pJR68 ↔ LMSW30 |
| LMSW120 | ΔreoM attB::Phelp-lacO-reoM R66A lacI neo | pSW55 → LMSW30 |
| LMSW121 | ΔreoM attB::Phelp-lacO-reoM R70A lacI neo | pSW56 → LMSW30 |
| LMSW123 | ΔreoM attB::Phelp-lacO-reoM T7A lacI neo ΔreoY | pSW29 → LMSW117 |
| LMSW124 | ΔreoM attB::Phelp-lacO-reoM T7A lacI neo ΔmurZ | pSW29 → LMSW119 |
| LMSW125 | ΔreoM attB::Phelp-lacO-reoM R57A lacI neo | pSW58 → LMSW30 |
| LMSW126 | ΔreoM attB::Phelp-lacO-reoM R62A lacI neo | pSW59 → LMSW30 |
| LMSW138 | ΔreoY attB::Phelp-lacO-reoY lacI neo | pJR70 → LMSW32 |
| LMSW139 | ΔmurZ attB::Phelp-lacO-murZ lacI neo | pJR71 → LMJR104 |
*The arrow (→) stands for a transformation event and the double arrow (↔) indicates gene deletions obtained by chromosomal insertion and subsequent excision of pMAD plasmid derivatives (see experimental procedures for details).