Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | pMBP-AsCas12a expression plasmid | Chen et al. (2018) | RRID:Addgene_113430 | See Supplementary file 1 for the sequences of all plasmids and oligonucleotides |
| Peptide, recombinant protein | T4 polynucleotide kinase | New England Biolabs | M0201S | |
| Peptide, recombinant protein | T4 RNA ligase 2 | New England Biolabs | M0239S | |
| Peptide, recombinant protein | AsCas12a protein | this paper | All Cas proteins purified from E. coli BL21 Star(DE3) cells | |
| Chemical compound, drug | [γ-32P]-ATP | PerkinElmer | BLU502A001MC | |
| Chemical compound, drug | potassium permanganate | Sigma-Aldrich | 223468 | |
| Software, algorithm | ImageQuant TL | GE Healthcare | RRID:SCR_014246 |