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. Author manuscript; available in PMC: 2020 Aug 13.
Published in final edited form as: Lab Invest. 2020 Feb 13;100(6):837–848. doi: 10.1038/s41374-020-0405-8

Figure 6: Evaluation of VEGF signaling via the H2HR/ pERK pathway after modulation of HDC/histamine axis.

Figure 6:

The expression of H2HR (A) and pERK (B) shown by qPCR and immunofluorescence, respectively increased in Mdr2−/− mice compared to WT, which was ablated in DKO mice. Treatment with histamine in DKO mice increased these parameters (A-B). The expression of vWF (C) shown by immunofluorescence increased in Mdr2−/− mice compared to WT, which was ablated in DKO mice. Treatment with histamine in DKO mice increased vWF expression (C). Both VEGF-R2 and VEGF-C expression increased in Mdr2−/− mice; however, in DKO mice, expression was lost (D). Again, treatment with histamine increased VEGF-R2 and VEGF-C expression in DKO mice. Data are mean ± SEM of n = 12 experiments for real-time PCR and n = 18 experiments for EIA. *p<0.05 vs. WT; #p<0.05 vs. Mdr2−/− mice and &p<0.05 vs. DKO. Representative images x20.