Table 2.

Apparent K_m values for C₅₅-lipid I-N^ε-C₆-dansylthiourea (2e-C₅₅) and UDP-GlcNAc at the different concentartions of the counterpart (UDP-GlcNAc or 2e-C₅₅)^a.

C55-lipid I-Nε-C6-dansylthiourea (2e-C55) concentration (μM)	Km of UDP-GlcNAc (μM)
37.5	36 ± 10b
75.0	37 ± 3.0
150	36 ± 5.0
225	36 ± 2.2
300	35 ± 8.1b
UDP-GlcNAc concentration (μM)	Km of C55-lipid I-Nε-C6-dansylthiourea (2e-C55) (μM)
46.9	13 ± 3.0b
62.5	25 ± 3.0
93.8	36 ± 5.0
187.5	39 ± 3.0
375.0	40 ± 5.0b

^aAll reactions were performed in the presence of MgCl₂ (50 μM)

^bHigher and lower concentrations were repeated three times.