Fig. 4.

GPR44 inhibition improves islet function and ameliorates secretion of proinflammatory cytokines in early phase post transplantation. (a–c) Human C-peptide (a), ratio of human proinsulin over insulin (b) and plasma levels of the human proinflammatory cytokines TNF-α and GRO-α (c) analysed on day 4 post transplantation in mice transplanted with human islets and treated with 60 mg/kg per day AZ8154 or vehicle. (d–f) Human HGF protein level (d) and mRNA expression of PDX-1 (e) and MAFA (f), normalised to RPL30, in transplanted human islet lysates obtained from mice treated with AZ8154 60 mg/kg per day or vehicle on day 4 post transplantation. For all analyses n = 14 mice/experimental group; data are presented as mean ± SD and analysed by Mann–Whitney U test. *p < 0.05 and **p < 0.01. D60, 60 mg/kg per day; hGRO-α, human GRO-α; hc-peptide, human C-peptide; hTNF-α, human TNF-α