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. 2020 Jun 10;40(6):BSR20193371. doi: 10.1042/BSR20193371

Figure 2. FXYD3 is the target gene of miR-342-3p.

Figure 2

(A) The effect of miR-342-3p mimics or inhibitor on miR-342-3p expression was estimated by RT-qPCR. (B) RT-qPCR and Western blot analyses were used to measure FXYD3 mRNA expression and protein level separately in transfected cells. (C) The potential miR-342-3p binding site in the sequence of FXYD3 was shown. (D) The effect of miR-342-3p mimics on the luciferase activity of FXYD3-WT reporter and FXYD3-Mut reporter was determined by luciferase reporter assay. (E and F) The interaction between FXYD3 and miR-342-3p was investigated by RIP and RNA pull-down assays; *P<0.05, **P<0.01, ***P<0.001.