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. 2020 Jun 10;40(6):BSR20193371. doi: 10.1042/BSR20193371

Figure 3. LINC01503 serves as a sponge for miR-342-3p and positively regulates FXYD3 expression.

Figure 3

(A) LINC01503 expression in CC tissues was identified by GEPIA2 database. (B) RT-qPCR was conducted to determine LINC01503 expression in CC cell lines. (C) The potential miR-342-3p binding site in the sequence of LINC01503 was shown. (D) The effect of miR-342-3p mimics on the luciferase activity of LINC01503-WT reporter and LINC01503-Mut reporter was estimated by luciferase reporter assay. (E and F) The interaction between LINC01503 and miR-342-3p was investigated by RIP and RNA pull-down assays. (G) The efficiency of LINC01503 overexpression and knockdown was assessed by RT-qPCR. (H) The effect of LINC01503 up-regulation and silence on the expression of miR-342-3p and FXYD3 was tested by RT-qPCR. (I) Western blot analysis was applied to detect the effect of LINC01503 overexpression and deficiency on the expression of FXYD3 protein; *P<0.05, **P<0.01.