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. 2020 Jun 10;10:9369. doi: 10.1038/s41598-020-66055-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro expansion and LIV synergistically decrease proteins associated with cytoskeleton and extracellular matrix. (a) 21 proteins relating to actin cytoskeleton were significantly decreased in either the LP or LP + LIV group. (b) Compared to P6, we have also identified six significantly downregulated microtubule related proteins, including three tubulin alpha and beta chains. (c) Extracellular matrix (ECM) related proteins, alpha-1 and/or apha-2 chains of Collagens I, V and XII were decreased by either LP or LP + LIV. n = 3/grp, group comparisons were made using one-way ANOVA followed by a Newman-Keuls post-hoc test. All presented proteins were p < 0.05 as identified via one-way ANOVA. Identified differences (p < 0.05) between LP and LP + LIV were further denoted as ¥: LP + LIV < LP, Ϯ: LP + LIV > LP.