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. 2020 Jun 10;10:9369. doi: 10.1038/s41598-020-66055-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mechanical activation of focal adhesion kinase was not altered by prolonged culture or LIV. MSCs were subjected to 2% equibiaxial strain at a rate of 0.2 Hz for 20 minutes and probed for Focal adhesion Kinase phosphorylation on Tyr397residue (pFAK). pFAK levels showed an 87% decrease (p < 0.05) in the LP group while LIV treated LP + LIV MSCs had a 300% increase in pFAK levels compared to the LP group. Upon strain application all three groups significantly increased pFAK levels compared to unstrained controls (p < 0.05) but were not statistically different from each other. n = 3/grp, group comparisons were made using one-way ANOVA followed by a Newman-Keuls post-hoc test. p < 0.05, **p < 0.01, ***p < 0.001, against control and each other. Full-length blots are presented in Supplementary Fig.S2.