Fig. 5. Structural transition from compacted to extended spirosome is important for AdhE activity.

a E817 and Q821 located at the inter-helical interface were mutated to cysteines to introduce cross-links to freeze the compact AdhE spirosome structure. b Negative stained EM analysis of the wild-type and Cys mutant AdhE spirosomes in the absence and presence of DTT. The scale bars show 50 nm. c A bar graph showing the relative activity of the wild-type (WT) and Cys mutant (Cys) in the absence (−DTT) and presence (+DTT) of DTT. Corresponding p values are 3.06 × 10⁻⁶ and 0.84, respectively. (****p value <0.0001, NS p value >0.05 from n = 3, two-tailed student’s t test).