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. 2020 Jun 10;10:9397. doi: 10.1038/s41598-020-66215-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The IHF binding site is operational in trans. Fraction of YFP-negative cells in cultures of E. coli strain 536, its isogenic intI⁻ mutant as well as in strain 536 intI⁻ complemented with a plasmid-encoded copy of intI containing the IHF binding site (pMC1) or complemented with a plasmid-encoded copy of intI lacking the IHF binding site (pMC2) in percent. Each of the columns represents the average of three biological replicates in which 10⁷ cells per sample were analyzed. (*P < 0.05; **P < 0.01; ***P < 0.001; schematic representation of the main features of pMC1 and pMC2 are shown below the graph).