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. 2020 Jun 10;10:9397. doi: 10.1038/s41598-020-66215-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Integrase I of E. coli 536 is subject to autoregulation and represses its own promoter. (A) YFP fluorescence signal of E. coli strains 536 intI::yfp (chromosomal promoter fusion), 536 intI-yfp (chromosomal operon), and E. coli strain 536 (pMC5) that encodes an artificial plasmid-encoded intI-yfp operon, and E. coli strain 536 (pMC3) with a plasmid-encoded intI::yfp fusion normalized to the cell density (OD₅₉₅). The columns represent the average of three biological replicates (*P < 0.05; **P < 0.01; ***P < 0.001). (B) YFP fluorescence signal distribution within the corresponding bacterial populations (10⁷ cells per sample were analyzed).