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. 2020 Jun 10;10:9397. doi: 10.1038/s41598-020-66215-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of the presence of the IHF binding site on intI promoter activity in E. coli strains 536 (pMC5) and 536 (pMC7). (A) Number of YFP-positive cells in the populations of E. coli strains 536 intI-yfp, 536 (pMC5) and 536 (pMC7). The columns represent the average of three biological replicates, in which 10⁷ cells per sample were analyzed (*P < 0.05; **P < 0.01; ***P < 0.001). (B) Distribution of the YFP fluorescence signal in the populations of E. coli strains 536 (pMC5) (grey) and 536 (pMC7) (black), which lacks the IHF binding site upstream of intI. In both populations 10⁷ cells were analyzed per sample.