Figure 3.

Analysis of intra- and extracellular annexin A5 levels in choroid plexus cultures after Aβ incubation. (a) Annexin A5 levels in epithelial choroid plexus cell cultures incubated with oligomerised Aβ₄₂ for 6, 12, 24 and 48 hours determined by Western blot. Annexin A5 levels are shown as percentage of untreated cells. β-actin was used as loading control. Histogram reveals a progressive decrease in annexin A5 levels in cells incubated with Aβ₄₂ already significant at 24 hours (upper panel) (**p < 0.01; n = 4). Representative bands of annexin A5 protein levels in choroid plexus cells at 0, 6, 12, 24 and 48 hours after Aβ₄₂ addition (bottom panel). These images were cropped from full-length blots and they are shown in the supplementary information section. In all cases data represents mean ± SEM. (b) Annexin A5 levels in extracellular medium of Aβ₄₂-incubated choroid plexus cells for 0, 6, 12, 24 and 48 hours. Histogram shows a progressive increase of annexin A5 levels in extracellular medium which is already significant 24 hours after Aβ₄₂ incubation (upper panel) (**p < 0.01; ***p < 0.001; n = 4). Representative bands of annexin A5 protein levels in extracellular medium at 0, 6, 12, 24 and 48 hours after Aβ₄₂ addition (bottom panel). This image was cropped from a full-length blot shown in the Supplementary Information section. In all cases data represents mean ± SEM.