Table 3.
Validation results for fortified equine urine samples (n = 26).
| No | Analyte | Transition (m/z) | eLODb (ng mL−1) | Cvalc (ng mL−1) | CCβ | Relative cut-off factor (RFm)d (%) | Sensitivitye (%) |
|---|---|---|---|---|---|---|---|
| 1 | AC-262536 | 279.2 > 195.0 | 0.03 | 1 | <Cval | 69 | 100 |
| 2 | Andarine (S-4)a | 440.2 > 150.0 | 0.06 | 2 | <Cval | 76 | 96 |
| 3 | Bicalutamidea | 429.2 > 255.0 | 0.02 | 1 | <Cval | 74 | 96 |
| 4 | BMS-564929 | 306.1 > 96.0 | 1.5 | 5 | <Cval | 66 | 100 |
| 5 | GLPG0492 | 390.2 > 118.0 | 0.09 | 1 | <Cval | 60 | 96 |
| 6 | LGD-2226 | 393.1 > 241.1 | 0.18 | 1 | >Cval | N/A | N/A |
| 7 | LGD-4033 | 337.1 > 267.2 | 0.007 | 1 | <Cval | 64 | 96 |
| 8 | Ly2452473 | 375.2 > 272.1 | 0.002 | 1 | <Cval | 17.2 | 96 |
| 9 | Ostarine (S-22)a | 388.1 > 118.0 | 0.004 | 1 | <Cval | 75 | 96 |
| 10 | PF-06260414 | 303.1 > 232.1 | 0.04 | 1 | <Cval | 69 | 100 |
| 11 | RAD140 | 394.1 > 223.1 | 0.05 | 1 | <Cval | 37.1 | 96 |
| 12 | S-1a | 401.1 > 261.1 | 0.01 | 1 | <Cval | 93 | 96 |
| 13 | S-6a | 435.1 > 145.0 | 0.21 | 1 | <Cval | 34.0 | 100 |
| 14 | S-9a | 417.2 > 261.2 | 0.08 | 1 | <Cval | 46.6 | 100 |
| 15 | S-23a | 415.2 > 145.0 | 0.11 | 1 | <Cval | 53 | 100 |
a
Values calculated response-based.
b
Estimated LOD (S/N≥3).
c
Screening target concentration.
d
Calculated as percentage based on the ratio of the cut-off factor and the mean response of fortified samples.
e
Expressed as percentage based on the ratio of samples detected as positive in true positive samples, following fortification.