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. 2020 Mar 30;18:1249–1258. doi: 10.1016/j.csbj.2020.03.019

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Growth curves: (a) Growth curves of mono- and co-cultures of P. stipitis and S. cerevisiae. These experiments were carried out in YNB (defined) medium. Here, represents the co-culture of P. stipitis and S. cerevisiae, represents the mono-culture of P. stipitis and represents the mono-culture of S. cerevisiae. Note that the growth curve of co-culture exhibits a diauxic pattern and lies between those of mono-cultures of P. stipitis and S. cerevisiae. (b) Comparison of the growth curve of P. stipitis grown on the supernatant of S. cerevisiae with that grown in control. This experiment was performed according to the scheme showed in Fig. 2. Here, represents control and represents experimental condition. Note the diauxic pattern of growth curve when P. stipitis is grown in the supernatant of S. cerevisiae. All the experiments were carried out at 30°C and 300 rpm. In both the cases, the growth was monitored online using CGQ (Aquila Biolabs). The experiments were performed in triplicates. Error bars represent the standard deviation values obtained from CGQuant software version 7.3. For more details on methods, see §2.2.3 and §2.2.4.

Inline graphic — Growth curves: (a) Growth curves of mono- and co-cultures of P. stipitis and S. cerevisiae. These experiments were carried out in YNB (defined) medium. Here, represents the co-culture of P. stipitis and S. cerevisiae, represents the mono-culture of P. stipitis and represents the mono-culture of S. cerevisiae. Note that the growth curve of co-culture exhibits a diauxic pattern and lies between those of mono-cultures of P. stipitis and S. cerevisiae. (b) Comparison of the growth curve of P. stipitis grown on the supernatant of S. cerevisiae with that grown in control. This experiment was performed according to the scheme showed in Fig. 2. Here, represents control and represents experimental condition. Note the diauxic pattern of growth curve when P. stipitis is grown in the supernatant of S. cerevisiae. All the experiments were carried out at 30°C and 300 rpm. In both the cases, the growth was monitored online using CGQ (Aquila Biolabs). The experiments were performed in triplicates. Error bars represent the standard deviation values obtained from CGQuant software version 7.3. For more details on methods, see §2.2.3 and §2.2.4.