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. 2020 May 27;23(6):101201. doi: 10.1016/j.isci.2020.101201

Figure 8.

Figure 8

Induction of Neural-like Phenotype by Locked Nucleic Acid (LNA) Ablation of miR-17-5p in UC6 Cell Line

(A) Detection of miR17-5p ablation by RT-qPCR induced by miR17-5p LNA with NegCtl LNA as control and miR-125a as an external reference.

(B) Top 50 up- and downregulated genes induced by miR17-5p LNA by RNA sequencing (RNA-seq). Genes highlighted in red are involved in neural differentiation.

(C) Representative miR17-5p target genes upregulated induced by miR17-5p LNA transfection revealed by RNA-seq.

(D) Representative upregulated urothelial and neural regulons induced by miR17-5p LNA transfection revealed by RNA-seq.

(E) Upregulation of E2F1 expression induced by miR17-5p LNA quantified by RT-qPCR.

(F) Representative upregulated E2F1 targets induced by miR17-5p LNA revealed by RNA-seq. In panels A and E, a p value < 0.05 was considered statistically significant.