Fig. 4.

Expression levels of protein related to mitochondrial fusion Mfn-1 (a) and Mfn-2 (b), fission Fis1 (c) and biogenesis TFAM (d) and NRF1 (e) in skeletal muscle of wild-type and transgenic animals fed ad libitum or under CR. General effects of diet and interaction between genotype and diet are represented, when detected, on the corresponding panel. Data are shown as mean ± SEM of 6 replicates except in c (Fis1) with 4 replicates. Western blots used for quantification of protein levels with their corresponding Ponceau S-stained lanes used for normalization of protein loading (f)