Protein acetylation was increased with aging. Representative Western blots (a) of total acetylated protein pool (b), acetylated PGC-1α (c), GCN5 (d), p65 (e), and SOD2 (f) in two skeletal muscles and cardiac muscle of young (N = 8), mid-aged (N = 8), and old mouse (N = 8), respectively. Data are mean ± SEM. *P < 0.05, **P < 0.01, Mid-aged or Old vs. Young; †P < 0.05, ††P < 0.01, old vs. mid-aged