Open field behavior of adult F0 (young = 7 months old, aged = 30 months old zebrafish developmentally exposed to THC, n = 8. a, b Male and female zebrafish distance traveled (cm). c, d Male and female velocity (cm/s). e, f Male and female mobility (%). Asterisk indicates a significant difference between aged and young controls (t test, p ≤ 0.05). Number sign indicates a significant difference compared with aged controls (ANOVA, Dunnett’s posthoc, p ≤ 0.05)