Fig. 3.

Weight, length, and spinal curvature (kyphosis) of adult F0 (young = 7 months old, aged = 30 months old) male (n = 8–22) & female (n = 5–12) zebrafish developmentally exposed to THC. a, b Male and female zebrafish total length (mm). c, d Male and female wet weight (g). e Male and female spinal curvature grading from healthy (1) to medium (3) to severe (5). f, g Male and female kyphosis (%). Asterisk indicates a significant difference between aged and young controls (t test, p ≤ 0.05). Number sign indicates a significant difference compared with aged controls (ANOVA, Dunnett’s posthoc, p ≤ 0.05 or Fisher’s exact test p ≤ 0.05)