Table 1.

F0 fecundity and survival of THC exposed (μM) zebrafish, data presented as mean ± SE

Nominal developmental treatment (μM) at 6 hpf	n	Avg no. of eggs per tank per week ± SE	% survival at 24 hpf ± SE	% hatch at 48 hpf ± SE	% hatch at 72 hpf ± SE	% survival at 96 hpf ± SE	Sperm/g testes*106 ± SE
Young control	6	491 ± 71.7*	66.2 ± 5	48.3 ± 5.3*	98.5 ± 1.4	62.8 ± 4.3	31.2 ± 3.1*
Aged control	6	73 ± 43	59.9 ± 6.6	18.3 ± 8.6	100 ± 0	56.4 ± 7.2	13.4 ± 1.5
0.08 THC	6	360.4 ± 101.0#	59.4 ± 4.7	39.9 ± 16.2	97.3 ± 1.9	53.2 ± 4.7	9.1 ± 1.5
0.4 THC	6	220 ± 42.1	42.1 ± 10.4	45.7 ± 14.5	95.8 ± 3.6	36.8 ± 9.7	5.9 ± 4.7
2 THC	6	92.8 ± 58.7	19.7 ± 3.8	22 ± 0.5	89.9 ± 7.6	16.1 ± 1.4#	N/A ± N/A

*p ≤ 0.05 t test, aged control vs young control

^#p ≤ 0.05 ANOVA, Dunnett’s post hoc vs aged control

F0: aged control = 30 months old, young control = 7 months old

n = number of breeding events (3 consecutive days of egg collection/week = 1 breeding event)