Table 2.
Male and female F0 (mean ± SE) THC exposed (μM) gene expression (log(2)ΔΔCt) relative to the aged controls, n = 6
| n | p16 ± SE | tnfα ± SE | il-1β ± SE | il-6 ± SE | pparα ± SE | pparγ ± SE | |
|---|---|---|---|---|---|---|---|
| Males | |||||||
| Young control | 6 | − 0.76 ± 0.61 | − 2.63 ± 0.72* | − 3.24 ± 0.30* | 1.03 ± 0.22* | 0.56 ± 0.42 | 1.46 ± 0.09* |
| Aged control | 6 | 0 ± 0.4 | 0 ± 0.78 | 0 ± 0.6 | 0 ± 0.16 | 0 ± 0.19 | 0 ± 0.23 |
| 0.08 THC | 6 | − 0.04 ± 0.04 | − 2.56 ± 0.45# | − 1.04 ± 0.26 | 0.78 ± 0.26# | 0.45 ± 0.39 | 0.48 ± 0.34 |
| 0.4 THC | 6 | 0.92 ± 0.26 | − 0.21 ± 0.50 | 0.29 ± 0.63 | 1.39 ± 0.19# | 1.98 ± 0.12# | 1.30 ± 0.34# |
| 2 THC | 6 | 1.05 ± 0.12 | 0.34 ± 0.46 | 0.8 ± 0.62 | 0.77 ± 0.28# | 0.96 ± 0.28 | 1.18 ± 0.38# |
| Females | |||||||
| Young control | 6 | − 1.13 ± 0.29 | − 4.27 ± 0.65* | − 2.84 ± 0.14* | − 0.76 ± 0.19* | 0.68 ± 0.54 | − 0.23 ± 0.38 |
| Aged control | 6 | 0 ± 0.43 | 0 ± 0.80 | 0 ± 0.56 | 0 ± 0.25 | 0 ± 0.31 | 0 ± 0.37 |
| 0.08 THC | 6 | 0.50 ± 0.24 | − 3.15 ± 0.57# | −2.14 ± 0.28# | − 0.06 ± 0.32 | 0.11 ± 0.23 | − 0.87 ± 0.20 |
| 0.4 THC | 6 | 0.31 ± 0.08 | − 2.37 ± 0.46 | − 1.98 ± 0.40# | 0.43 ± 0.35 | 0.16 ± 0.42 | − 1.00 ± 0.24 |
| 2 THC | 6 | 0.01 ± 0.26 | −1.31 ± 0.71 | −1.60 ± 0.42 | 0.65 ± 0.24 | 0.26 ± 0.32 | −1.37 ± 0.25# |
*p ≤ 0.05 t test, aged control vs young control
#p ≤ 0.05 ANOVA, Dunnett’s post hoc vs aged control
Exposure groups were normalized to 18 s, and the ΔCT values were assessed for significance by one-way ANOVA with Dunnett’s post hoc test (p ≤ 0.05), or by t test for aged compared with young (p ≤ 0.05)