Table 3.
Male and female F1 (mean ± SE) gene expression (log(2)ΔΔCt) of fish (parents exposed to 2 μM THC) relative to the aged controls, n = 6
| n | p16 ± SE | tnfα ± SE | il-1β ± SE | il-6 ± SE | pparα ± SE | pparγ ± SE | |
|---|---|---|---|---|---|---|---|
| Males | |||||||
| Aged control | 6 | 0 ± 0.59 | 0 ± 0.9 | 0 ± 0.43 | 0 ± 0.16 | 0 ± 0.09 | 0 ± 0.19 |
| 2 μM THC | 6 | 0.4 ± 0.82 | 0.89 ± 0.85 | − 0.45 ± 0.58 | − 0.62 ± 0.62 | − 0.52 ± 0.59 | − 0.64 ± 0.52 |
| Females | |||||||
| Aged control | 6 | 0 ± 50 | 0 ± 0.57 | 0 ± 0.52 | 0 ± 0.26 | 0 ± 0.48 | 0 ± 0.18 |
| 2 μM THC | 6 | − 0.51 ± 0.55 | 0.13 ± 0.77 | − 0.86 ± 1.27 | − 0.02 ± 0.56 | − 0.92 ± 0.88 | − 1.13 ± 0.70 |
p >0.05 ANOVA, t test vs aged control
Exposure groups were normalized to 18 s and the ΔCT values were assessed for significance by t test (p ≤ 0.05)