Fig. 2.

Analysis of the senescence-associated secretory phenotype in parabiotic mice. A Total RNA was isolated from snap-frozen tissues collected from parabiotic mice (n = 5–8 mice per group). Expression of senescence-associated secretory phenotype (SASP) genes Il1β, Il6, Mcp1, and Tnfα was measured by qPCR using the ∆∆Ct method and normalized to Gapdh expression. B Hepatic and renal levels of the SASP factor MCP-1 were quantified by ELISA (n = 7 mice per group). Values represent the mean ± SD and one-way ANOVA with Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001