Fig. 1.

Illustration showing the transcription start site (TSS) and upstream regulatory regions of the human CYP2E1 and the homologous mouse Cyp2e1 gene. Upstream regulatory element positions were obtained from GeneHancer (Fishilevich et al. 2017) and ORegAnno (Lesurf et al. 2016). DNA methylation assays were conducted in the current study at both the TSS and upstream regulatory region in mouse at positions marked by the arrows. Reference histone 3 lysine 9 acetylation (H3K9ac) and histone 3 lysine 27 acetylation (H3K27ac) in 8-week-old mouse livers are from the ENCODE/LICR track in UCSC Genome Browser. Two loci with high liver histone acetylation levels were chosen for analysis using ChIP-qPCR in the current study, at positions marked by the arrows. For exact assay coordinates, see Supplementary Tables S2 and S3