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. 2020 Jun 4;14:28. doi: 10.3389/fnana.2020.00028

FIGURE 7.

FIGURE 7

CAR expression in the midbrain and brainstem. CAR immunoreactivity in brown counterstained with Harry’s hematoxylin in blue. (A) Coronal section of mouse brain; CAR is present in the neuropil of the superior colliculus, the periaqueductal gray, and in the interpeduncular nucleus. (B) High magnification of (A) showing CAR immunostaining in the neuropil of the zonal layer in the superior colliculus. (C) Coronal section of mouse brain; CAR is present in the neuropil of the superior colliculus, the periaqueductal gray, and in the midline nuclei of raphe and reticular nuclei. (D) High magnification of (C) showing the presence of CAR in the neuropil of the midline raphe nuclei. (E) High magnification of (C) showing CAR immunoreactivity in the neuropil of the midline, corresponding to the tegmental reticular nucleus and raphe magnus. (F) Coronal section of mouse brain showing the presence of CAR immunostaining in the neuropil of the molecular layer of the cerebellar cortex, in the medial vestibular nucleus and dorsal cochlear nucleus. (G) High magnification of (F) showing the pattern of expression of CAR in the dorsal cochlear nucleus. (H) Coronal section of mouse brain showing CAR expression in the caudal rhombencephalon, in the area postrema, and inferior olive complex. (I) High magnification of (H) showing the presence of CAR immunostaining in the neuropil of the inferior olive complex. (J) Sagittal section of a mouse brain showing the presence of CAR immunolabeling in the superior colliculus, interpeduncular nucleus, in the dorsal tegmental nucleus, area postrema, inferior olive complex and raphe nuclei. (K) High magnification of (J) showing the presence of CAR in the neuropil of the dorsal tegmental nucleus and the ependymal cell lining the 4th ventricle (arrows). (L) Magnification of (J) showing the 4th ventricle and the area postrema. CAR is present in the neuropil and in the projections toward the parenchyma. Calibration bars: (A–B): 1 mm; (C–E): 5 μm; (F): 1 mm; (G): 5 μm; (H): 1.5 mm; (I): 5 μm; (J): 1 mm; (K): 5 μm; (L): 15 μm.