Table 2.
Table of primer and probe sequences for detecting SARS-CoV-2 genes.
| Country | Institute | Gene target | Sequence | References |
|---|---|---|---|---|
| China | China CDC | ORF1ab | F: CCCTGTGGGTTTTACACTTAA | Ledsgaard et al., 2018 |
| R: ACGATTGTGCATCAGCTGA | ||||
| P: 5′-FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3′ | ||||
| N | F: GGGGAACTTCTCCTGCTAGAAT | |||
| R: CAGACATTTTGCTCTCAAGCTG | ||||
| P: 5′-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3′ | ||||
| Germany | Charité | RdRP | F2: 5′-GTGARATGGTCATGTGTGGCGG-3′ | Chu et al., 2020 |
| R1: 5′-CARATGTTAAASACACTATTAGCATA-3′ | ||||
| P2: 5′-FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ-3′ | ||||
| P1: 5′-FAMCCAGGTGGWACRTCATCMGGTGATGC-BBQ-3′ | ||||
| E | F1: 5′-ACAGGTACGTTAATAGTTAATAGCGT-3′ | |||
| R2: 5′-ATATTGCAGCAGTACGCACACA-3′ | ||||
| P1: 5′-FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ-3′ | ||||
| Hong Kong SAR | Hong Kong University | ORF1b | F: 5′-TGGGGYTTTACRGGTAACCT-3′ | World Health Organization, 2020b |
| R: 5′-AACRCGCTTAACAAAGCACTC-3′ | ||||
| P: 5′-FAM-TAGTTGTGATGCWATCATGACTAG-TAMRA-3′ | ||||
| N | F: 5′-TAATCAGACAAGGAACTGATTA-3′ | |||
| R: 5′-CGAAGGTGTGACTTCCATG-3′ | ||||
| P: 5′-FAM-GCAAATTGTGCAATTTGCGG-TAMRA-3′ | ||||
| Japan | National Institute of Infectious Diseases, Depart of Virology III | N | F: 5′-AAATTTTGGGGACCAGGAAC-3′ | Nie et al., 2020 |
| R: 5′-TGGCAGCTGTGTAGGTCAAC-3′ | ||||
| P: 5′-FAM-ATGTCGCGCATTGGCATGGA-BHQ-3′ | ||||
| Thailand | National Institute of Health | N | F: 5′-CGTTTGGTGGACCCTCAGAT-3′ | Notomi et al., 2000 |
| R: 5′-CCCCACTGCGTTCTCCATT-3′ | ||||
| P: 5′-FAM-CAACTGGCAGTAACCABQH1-3′ | ||||
| USA | US Center of Disease Control and Prevention | N | F: 5′-GAC CCC AAA ATC AGC GAA AT-3′ | Li J. et al., 2018 |
| R: 5′-TCT GGT TAC TGC CAG TTG AAT CTG-3′ | ||||
| P: 5′-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3′ | ||||
| F: 5′-TTA CAA ACA TTG GCC GCA AA-3′ | ||||
| R: 5′-GCG CGA CAT TCC GAA GAA-3′ | ||||
| P: 5′-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3′ | ||||
| F: 5′-GGG AGC CTT GAA TAC ACC AAA A-3′ | ||||
| R: 5′-TGT AGC ACG ATT GCA GCA TTG-3′ | ||||
| P: 5′-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3′ | ||||
| RP-F: 5′-AGA TTT GGA CCT GCG AGC G-3′ | ||||
| RP-R: 5′-GAG CGG CTG TCT CCA CAA GT-3′ | ||||
| RP-P: 5′-FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1-3′ | ||||
| France | Institut Pasteur | RdRP | F: 5′-ATGAGCTTAGTCCTGTTG-3′ | Vincent et al., 2004 |
| R: 5′-CTCCCTTTGTTGTGTTGT-3′ | ||||
| P: 5′-AGATGTCTTGTGCTGCCGGTA [5′]HEX [3′]BHQ-1-3′ | ||||
| F: 5′-GGTAACTGGTATGATTTCG-3′ | ||||
| R: 5′-CTGGTCAAGGTTAATATAGG-3′ | ||||
| P: 5′-TCATACAAACCACGCCAGG [5′]FAM [3′]BHQ-1-3′ | ||||
| E | F: 5′-ACAGGTACGTTAATAGTTAATAGCGT-3′ | |||
| R: 5′-ATATTGCAGCAGTACGCACACA-3′ | ||||
| P: 5′-ACACTAGCCATCCTTACTGCGCTTCG [5′]FAM [3′]BHQ-1-3′ |