FIGURE 7.

Effects of different treatments used in clinics on the morphological characteristics of IBD organoids. IBD organoids were derived from IBD patients. Crohn’s disease (CD) patient organoids were represented with closed symbols and keyboard symbols, while ulcerative colitis (UC) patient organoids were represented by opened symbols. One patient was represented by the same symbol in all figure panels. IBD organoids were treated from day 0 to day 10 with anti-TNF (Remicade) at 1, 10, and 100 μM, or methyl-prednisolone (Solupred) at 10 and 100 μM, or 5-ASA (Pentasa) at 50 and 500 μM. Where possible, an average of 20 organoids was studied per condition per patient. Medians were represented by red lines on graphs. Doted lines represented for each criteria, the median measured for this criteria in control (non-IBD), non-treated organoid cultures. Statistics were not performed on these graphs due to the low n number in some groups. (A) Average size of organoids (μm) (B) Number of colonoids (budding structures) per total number of organoids (colonospheres plus colonoïds) (C) Number of organoids with empty lumen per total number of organoids (empty, dense and without lumen). (D) Number of inverted organoids per total number of organoids (oriented plus inverted).