Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 10;10:9414. doi: 10.1038/s41598-020-66120-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Tumor infiltration promoted by crosstalk between MFS cells and NFs. (A) Schema of invasion assay using MFS cells and normal fibroblasts educated by tumor-derived EVs or synthetic miR-1260b/control. (B) Fluorescence microscope shows PKH-67-labeled EVs derived from MFS were captured in NFs. Scale bar = 100 nm. (C) Upregulated miR-1260b levels in NFs educated using MFS-derived EVs. **p < 0.01; Student’s t test. (D) Invasion assay. Left, NMFH-1; right, NMFH-2. EV-educated NFs derived from MFS cells promoted cellular invasion of MFS cells. ***p < 0.001; Student’s t test. (E) Upregulated miR-1260b levels in NFs transfected with synthetic miR-1260b mimic or control. ***p < 0.001; Student’s t test. (F) Invasion assay. Left, NMFH-1; right, NMFH-2. NFs upregulated with miR-1260b mimic promoted cellular invasion of MFS cells. ***p < 0.001; Student’s t test. (G) Schema of invasion assay using MFS cells transfected with synthetic miR-1260b or control. (H) Invasion assay. Left, NMFH-1; right, NMFH-2. Scale bar = 100 μm. N.S., not significant; Student’s t test.