Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 10;11:2936. doi: 10.1038/s41467-020-16747-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Schematic depiction of the UPR-induced enzymatic pathway that diverts intermediary metabolites from glycolysis to fuel mitochondrial one-carbon metabolism via serine biosynthesis. PHGDH: phosphoglycerate dehydrogenase, PSAT1: phosphoserine aminotransferase 1, PSPH: phosphoserine phosphatase, SHMT2: serine hydroxymethyltransferase 2, MTHFD2: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ALDH1L2: aldehyde dehydrogenase 1 family member L2, THF: tetrahydrofolate, 5,10-meTHF: 5,10-methylene THF. b Analysis of the redox status of the nicotinamide adenine dinucleotide phosphate pool in LN-308 cells after 24 h of control treatment with DMSO (yellow bar), or treatment with TM (green bar). P-value: 0.0003. c Analysis of the cellular concentration of the amino acids serine, proline, and glycine after induction of the UPR (green bars) relative to control treatment with DMSO (yellow bars). Fold-increase values after 24 h of treatment are displayed at the top of each panel. d Analysis of the isotope distribution of serine and glycine 24 h after application of a metabolically labeled serine tracer. Cells were either control treated with DMSO (yellow bars) or stimulated for 24 h with TM (green bars). P-values: Serine M + 7: 0.590, Glycine M + 4: 0.001. e Analysis of the contribution of mitochondrial folate metabolism to thymidylate synthesis in control-treated LN-308 cells (yellow bars), or after induction of the UPR with TM (green bars). The isotope distribution of dTTP was analyzed 24 h after administration of a metabolically labeled serine tracer. P-values: dTTP M + 0: 0.713, dTTP M + 2: 0.038, and dTTP M + 3: 0.002. All data are represented as mean ± SD of three (c–e) or four (b) biologically independent experiments. A Student’s t-test was used for the statistical testing with the following parameters: two-sided, true variance equality and confidence level at 0.95. No adjustment for multiple comparisons was made. n.s. not significant P > 0.05, *P < 0.05, ***P < 0.005. Source data are provided as a Source Data file.