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. 2020 Jun 10;10:9449. doi: 10.1038/s41598-020-65941-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of CCMP470 under LM (A,B), SEM (C,E) and TEM (D,F,G). (A) Chain colony under bright field, and (B) under phase contrast. (C) Frustule in oblique view, showing numerous girdle bands. Notice the slightly wrinkled appearance of the bands even after preparation by critical-point drying, indicating weak silicification of frustule. (D) Collapsed theca with >10 girdle bands. Each band has a primary rib from which secondary ribs extend perpendicularly. The inset is an enlargement of the part marked by an asterisk, showing the primary and secondary ribs of a band (arrowhead and double arrowhead, respectively). (E–G) Circular valve with a distinct sternum. Virgae extend perpendicularly from the sternum but become radiate towards the periphery of the valve. Occasional bifurcation takes place to keep the stria density even throughout valve face (indicated by arrows), except for valve apices which display a distinct region with slightly finer striae; these most likely represent apical fields responsible for mucilage secretion. The highly reduced labiate process formed along the sternum can be inconspicuous (E), transapically elongated (F), or double (G). Scales = 5 µm (A,B) and 1 µm (C–G).