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. 2020 Jun 10;10:9449. doi: 10.1038/s41598-020-65941-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evolutionary position of CCMP470 based on BUSCO sequences. A. Consensus topology of a 106 taxa × 16,774 aa alignment of 35 BUSCOs conserved in published diatom genome and transcriptome libraries, inferred with MrBayes and RAxML using three substitution matrices (MrBayes: GTR, Jones, WAG; RAxML: GTR, JTT, WAG). The tree topology is rooted on four evolutionary outgroup ochrophyte genomes (in grey). Diatom taxa are shaded by evolutionary origin, and Plagiostriata CCMP470 is shown in black. The topology shown is the consensus MrBayes topology; alternative phylogenetic positions for CCMP470, as inferred using Bayesian and RAxML analysis, are shown with labelled circles.