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. 2020 Jun 10;11(6):446. doi: 10.1038/s41419-020-2655-1

Fig. 7. Bmal1 activates transcription of Alb, Hp, and Tf through direct promoter occupancy.

Fig. 7

a Reporter gene assays in HK-2 cells transfected with indicated plasmids for 24 h, and then treated with 20 μM cisplatin or vehicle for another 24 h. n = 6, *P < 0.05 and **P < 0.01 vs. vector group, ##P < 0.01 vs. Bmal1 overexpression or cisplatin alone group. b Reporter gene assays in HK-2 cells with Bmal1 knockdown. n = 6, **P < 0.01 vs. scramble shRNA group, ##P < 0.01 vs. scramble shRNA + cisplatin group. c ChIP assays with indicated antibodies in HK-2 cells similarly treated as described in Fig. S2. The enrichments were quantified by RT-qPCR analysis. n = 3, **P < 0.01 vs. Ad-GFP group, #P < 0.05 and ##P < 0.01 vs. Ad-GFP + cisplatin group. d ChIP assays with indicated antibodies in HK-2 cells similarly treated as described in Fig. S5. The enrichments were quantified by RT-qPCR analysis. n = 3, **P < 0.01 vs. scramble shRNA group, #P < 0.05 and ##P < 0.01 vs. scramble shRNA + cisplatin group. All values are presented as the mean ± SD.