Fig. 5. Longevity and behavioral decline across thermal perturbations.

a Lifespan curves and corresponding dot plot for wild-type N2 populations cultured at food level OD₆₀₀5 at constant 15 °C (36.21 days ± 1.19, n = 71 biologically independent animals), 17.5 °C (31.60 ± 1.09, n = 107 biologically independent animals), and 20 °C (28.65 ± 0.80, n = 77 biologically independent animals), and under oscillatory temperature conditions, changing from 15 °C to 20 °C every 12 h (29.10 ± 1.04, n = 99 biologically independent animals). Error is reported as SEM. (log-rank test, 20 °C v. 17.5 °C p = 0.0094, 20 °C v. 15 °C p < 0.001, 20 °C v. 15 °C ↔ 20 °C p = 0.2082, 17.5 °C v. 15 °C p = 0.0574, 17.5 °C v. 15 °C ↔ 20 °C p = 0.1371, 15 °C v. 15 °C ↔ 20 °C p = 0.0004). b Heatmaps showing individual decline in raw movement across static temperature conditions over time. c Population averaged raw movement for the static temperature conditions and relative population average activity for raw movement normalized to lifespan across static temperature conditions. Error bars are SEM. d Duration of high activity for raw movement across temperatures and relative fraction of life in high activity for raw movement across temperature conditions. Error bars are SD. (**p < 0.01, ***p < 0.001, via one-way ANOVA followed by Tukey’s HSD test. Non-significant differences are not marked). e (i) Heatmap showing individual decline in raw movement across oscillatory temperature condition over time. (ii) Population averaged raw movement activity for the oscillatory temperature condition. Error bars are SEM. (iii) Average raw movement in oscillatory condition separated by temperature the population was experiencing at the time. Error bars are SEM.