Table 11.

The 80X mapped dataset.

DNA fragment length	Mapped coverage (X)	%1X	%5X	%10X	%20X	%30X	% PASS	% PASS RD > 10	Fold enrichment	FOLD 80 penalty
200	80.72	99.86	99.82	99.71	98.42	95.21	95.55	95.45	43.50	1.53
230	80.00	99.86	99.81	99.65	97.94	94.22	95.52	95.36	45.67	1.58
260	79.99	99.81	99.73	99.68	99.54	99.13	96.38	96.34	35.13	1.34
270	79.97	99.79	99.72	99.67	99.54	99.13	96.40	96.37	34.72	1.34
280	80.00	99.79	99.71	99.65	99.48	99.01	96.43	96.38	33.79	1.36
290	80.02	99.80	99.71	99.65	99.49	99.01	96.43	96.38	33.10	1.36
340	79.99	99.84	99.78	99.71	99.50	98.88	96.53	96.47	31.59	1.37
360	79.96	99.84	99.78	99.70	99.08	97.11	96.58	96.51	33.52	1.42
400	80.05	99.84	99.78	99.69	98.93	96.66	96.58	96.50	32.17	1.45

For each DNA fragment length, the 80 mapped X-fold coverage is shown for the Twist target design dataset (mean of three independent experiments). The columns show the mapped coverage on the target, percentage of the target covered by at least 1, 5, 10, 20 and 30 reads, percentage of callable bases on the target for standard read depth (>3) and read depth >10, fold enrichment and FOLD 80 base penalty.