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. 2020 Jun 10;10:9424. doi: 10.1038/s41598-020-66331-z

Table 2.

The 80X mapped dataset.

ID Mapped coverage (X) %1X %5X %10X %20X %30X % PASS % PASS RD > 10 Fold enrichment FOLD 80 penalty
IDT-S 78.04 99.83 99.77 99.67 98.72 95.26 96.81 96.71 49.99 1.60
IDT-M 80.00 99.82 99.72 99.45 97.01 90.60 97.62 97.32 40.49 1.93
IDT-L 80.29 99.82 99.63 98.89 93.99 85.11 97.55 96.73 37.28 2.28
Roche-S 80.30 99.88 99.55 98.96 96.71 91.89 96.29 95.61 35.46 1.86
Roche-M* 66.83 99.85 99.40 98.54 94.63 86.06 96.99 96.01 33.74 1.91
Roche-L 79.52 99.85 99.49 98.83 95.99 89.65 97.04 96.30 28.82 2.01
Agilent-S 77.73 99.65 99.15 98.25 94.79 88.17 97.58 96.58 32.79 2.05
Agilent-M 80.01 99.64 99.30 98.76 96.26 90.63 98.23 97.64 30.29 1.95
Agilent-L 76.42 99.66 99.27 98.55 94.99 87.51 98.20 97.39 26.82 2.05
Twist-S 80.00 99.86 99.81 99.65 97.94 94.22 95.52 95.36 45.67 1.58
Twist-M 79.96 99.84 99.78 99.70 99.08 97.11 96.58 96.51 33.52 1.42
Twist-L 80.05 99.84 99.78 99.69 98.93 96.66 96.58 96.50 32.17 1.45

For each platform and DNA fragment length combination, the 80 mapped X-fold coverage is shown for the target design dataset (mean of the three independent experiments). The columns show the mapped coverage on the target, percentage of the target covered by at least 1, 5, 10, 20 and 30 reads, percentage of callable bases on the target for standard read depth (>3) and read depth >10, fold enrichment and FOLD 80 base penalty. DNA fragment lengths: S = short, M = medium, L = long.

*The sequencing data available for this combination did not reach 80X mapped coverage.