Table 5.

Downsampled mapped coverage for the platform showing the most beneficial effect of longer DNA fragments on FOLD 80 values.

Mapped coverage (X)	%1X	%5X	%10X	%20X	%30X	% PASS	% PASS RD > 10	Fold enrichment	FOLD 80 penalty
Twist-S
80	99.86	99.81	99.65	97.94	94.22	95.52	95.36	45.67	1.58
70	99.86	99.79	99.52	97.02	92.09	95.51	95.25	45.68	1.58
60	99.86	99.77	99.28	95.56	88.66	95.50	95.01	45.67	1.60
50	99.85	99.71	98.80	93.13	82.65	95.48	94.53	45.67	1.60
40	99.84	99.56	97.74	88.39	70.86	95.41	93.50	45.67	1.62
30	99.83	99.09	95.29	77.32	47.59	95.18	91.09	45.67	1.66
20	99.78	97.36	87.52	47.91	13.60	94.22	83.48	45.67	1.71
10	99.30	86.10	48.97	4.46	0.59	87.35	45.76	45.67	1.88
Twist-M
80	99.84	99.78	99.70	99.08	97.11	96.58	96.51	33.52	1.42
70	99.84	99.77	99.66	98.66	95.67	96.57	96.47	33.51	1.42
60	99.83	99.76	99.58	97.87	93.03	96.57	96.40	33.52	1.42
50	99.83	99.73	99.40	96.32	87.50	96.55	96.21	33.51	1.44
40	99.82	99.68	98.94	92.62	74.75	96.53	95.77	33.51	1.46
30	99.81	99.49	97.55	81.67	47.28	96.45	94.39	33.51	1.50
20	99.79	98.64	91.45	47.98	11.39	96.02	88.26	33.51	1.58
10	99.54	89.28	49.25	3.28	0.38	90.86	46.27	33.51	1.58

Parameters were calculated on 10–80X downsampled sets, including mapped coverage on the target, percentage of the target covered by at least 1, 5, 10, 20 and 30 reads, percentage of callable bases on the target for standard read depth (>3) and read depth >10, fold enrichment and FOLD 80 base penalty.