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. 2020 Jun 10;10:9400. doi: 10.1038/s41598-020-66417-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of infiltrated monocytes in the brain induced by pamoic acid (PA). The representative, flowcytometry diagram shows the Ly6C^Lo and Ly6C^Hi subsets of monocyte (C,D,G,H) gated from the CD45⁺ CD11b⁺ cell population (A, B, E, and F) in the ischemic hemisphere at both 24 h and 48 h after the MCAO respectively. I, J. PA treatment increased Ly-6C ^Lo cell population 24 h after the MCAO. *P = 0.0175, (unpaired t-test, n = 4). However, the Ly-6C ^Hi cell population was unaffected by PA treatment. K, L. PA treatment increased Ly-6C ^Lo cell population 48 h after the MCAO. *P = 0.0187, (unpaired t-test, n for MCAO = 5, n for MCAO + PA = 6). Ly-6C ^Hi cell population was unaffected by PA treatment at this time point.