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. 2020 Jun 10;10:9400. doi: 10.1038/s41598-020-66417-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pamoic acid (PA) treatment reduced the infiltration of GPR35 expressing neutrophil in the ischemic brain. The representative flow cytometry diagram shows the CD45 gated (A,B,E,F) Ly6G^Hi CD11b^Hi cells (C,D,G,H) in the ischemic hemisphere at both 24 h and 48 h after the MCAO respectively. These neutrophils (Ly6G^Hi-CD11b^Hi) also expressed GPR35 (J). I. PA treatment reduced infiltrating neutrophils 24 h after the MCAO. *P = 0.0408, (unpaired t-test), values are means ± s.e.m, n = 5. K. PA treatment reduced infiltrating neutrophils 48 h after the MCAO. *P = 0.0277, (unpaired t-test), values are means ± s.e.m, n = 5.