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. 2020 Jun 10;10:9426. doi: 10.1038/s41598-020-66442-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental methodology for the characterization of Ig heavy and light chains detected in control and KC tears. Tears were collected from non-KC control individuals (n = 7) and KC patients (n = 7) using passive tear collection from the lateral tear meniscus into a glass capillary tube followed by proteomic analysis and immunoglobulin (Ig) characterization. The relative distributions of Ig α, Ig λ, Ig κ, and Ig µ were plotted for each individual. Pictorials modified from Servier Medical Art under a Creative Commons Attribution 3.0 Unported License available at smart.servier.com.