Table 1.
The distribution of immunoglobulin (Ig) heavy and light chains detected in tears isolated from control individuals and KC patients.
| Ig Factor | Chain | Ig Isotype | Control Individuals | KC Patients | ||
|---|---|---|---|---|---|---|
| Relative abundance + Stdev (LFQ Intensity) | % of patients with detectable expression | Relative abundance + Stdev (LFQ Intensity) | % of patients with detectable expression | |||
| Ig α-1 chain C | heavy | IgA |
6.76E + 08± 6.90E + 08 |
57% |
6.26E + 08± 6.78E + 08 |
57% |
| Ig α-2 chain C | heavy | IgA |
4.02E + 08± 5.41E + 08 |
100% |
2.32E + 08± 4.21E + 08 |
71% |
| Ig λ-7 chain C | light | IgG, IgA, IgM, IgD, IgE |
7.00E + 04± 1.85E + 05 |
14% |
6.51E + 05± 1.48E + 06 |
43% |
| Ig λ chain V-IV | light | IgG, IgA, IgM, IgD, IgE |
1.89E + 06± 2.37E + 06 |
43% |
1.03E + 06± 1.77E + 06 |
29% |
| Ig κ chain C | light | IgG, IgA, IgM, IgD, IgE |
1.21E + 08± 1.21E + 08 |
71% |
3.62E + 07± 5.29E + 07 |
43% |
| Ig κ chain V-I | light | IgG, IgA, IgM, IgD, IgE |
2.37E + 07± 6.26E + 07 |
14% | 5.00E + 06 + 6.88E + 06 | 43% |
| Ig κ chain V-II | light | IgG, IgA, IgM, IgD, IgE |
1.23E + 09± 1.60E + 09 |
43% | 2.35E + 08 + 3.22E + 08 | 71% |
| Ig κ chain V-III (a) | light | IgG, IgA, IgM, IgD, IgE |
1.74E + 06± 1.71E + 06 |
57% |
0.00E + 00± 0.00E + 00 |
0% |
| Ig κ chain V-III (b) | light | IgG, IgA, IgM, IgD, IgE |
2.95E + 07± 7.31E + 07 |
29% |
5.15E + 06± 5.99E + 06 |
57% |
| Ig κ chain V-III (c) | light | IgG, IgA, IgM, IgD, IgE |
1.64E + 07± 3.89E + 07 |
71% |
0.00E + 00± 0.00E + 00 |
0% |
| Ig κ chain V-IV | light | IgG, IgA, IgM, IgD, IgE |
6.07E + 05± 1.61E + 06 |
14% |
1.97E + 06± 2.51E + 06 |
57% |
| Ig μ chain C | heavy | IgM |
3.19E + 07± 3.05E + 07 |
100% |
4.66E + 07± 5.97E + 07 |
100% |
| Ig heavy chain V-I | heavy | IgG, IgA, IgM, IgD, IgE | 1.06E + 06 + 1.37E + 06 | 43% |
1.38E + 05± 3.65E + 05 |
14% |
| Ig heavy chain V-II | heavy | IgG, IgA, IgM, IgD, IgE |
0.00E + 00± 0.00E + 00 |
0% |
4.64E + 05± 6.01E + 05 |
43% |
| Ig heavy chain V-III (d) | heavy | IgG, IgA, IgM, IgD, IgE |
0.00E + 00± 0.00E + 00 |
0% |
1.05E + 06± 1.49E + 06 |
43% |
| Ig heavy chain V-III (e) | heavy | IgG, IgA, IgM, IgD, IgE |
4.58E + 06± 6.31E + 06 |
43% |
5.00E + 06± 6.26E + 06 |
57% |
Specific regions identified separately within the same chain are reported here: (a) Ig κ chain V-III region VG; (b) Ig κ chain V-III region HAH; Ig κ chain V-III region HIC; Ig κ chain V-III region SIE; Ig κ chain V-III region WOL; Ig κ chain V-III region Ti; Ig κ chain V-III region GOL; (c) Ig κ chain V-III region CLL; Ig κ chain V-III region VH; Ig κ chain V-III region POM;Ig κ chain V-I region Lay; (d) Ig heavy chain V-III region VH26; (e) Ig heavy chain V-III region BRO;Ig heavy chain V-III region TEI;Ig heavy chain V-III region BUT;Ig heavy chain V-III region WEA.