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. 2020 Jun 10;10:9420. doi: 10.1038/s41598-020-66113-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nitric oxide inhibition attenuates CD4⁺ T-cell infiltration in the cerebellum following repetitive blast. (a) Flow cytometry revealed that repetitive TBI significantly increased CD4⁺ counts in the cerebellum (**p ≤ 0.01), which was significantly attenuated by L-NAME administration (*p ≤ 0.05). (b) No differences were measured in cerebellar CD8⁺ counts after repetitive TBI (p > 0.05). (c) Scatter plots for cerebellum of shams, (d) blast vehicle-treated, and (e) blast L-NAME-treated mice. (f) No differences in CD4⁺ counts (p > 0.05), or (g) CD8⁺ counts were measured in forebrain after repetitive mTBI (p > 0.05). (h) Scatter plots for forebrain of shams, (i) blast + vehicle-treated, and (j) blast + L-NAME-treated mice. One-way ANOVA post-hoc Newman-Keuls. Values represent means ± SEM.